Total coliform and E. coli, presence or absence measurement context
Standard Methods 9223 B, enzyme substrate: the sample is incubated with a medium containing substrates that total coliforms break down to a yellow color (ONPG) and that E. coli breaks down to a product that fluoresces under ultraviolet light (MUG). The result is present or absent in the volume tested, usually 100 mL. This is the usual test for drinking water and private wells.
Plan collection and receipt together
Define the organism or indicator, test portion and enumeration or presence/absence question. Use the assigned bottle and collection instructions and coordinate delivery around the method requirements.
Include the complete project requirement
Provide the matrix, target analytes, required method, fraction, units and reporting limits. Include the permit or monitoring instructions where results will support a regulatory submission. Naming this method does not establish blanket compliance eligibility.
Resolve collection before sampling
Use the bottle, preservation and delivery instructions assigned to the agreed scope. Identify each location and collection time, and document any deviation. The final analytical plan must fit the submitted material and its intended use.
Related testing & guidance
Technical & service references
Vanguard service detailsSource review: November 8, 2026. The agreed method and project scope govern the testing.