Testing by Vanguard Laboratory · Olympia, Washington360-967-7010

Bacteria & microbiology

SM 9221 C: Fecal coliform counts

Scope SM 9221 C for fecal coliform counts. Define sample matrix, preparation, reporting limits and project requirements.

Fecal coliform counts measurement context

Fecal (thermotolerant) coliforms grow at an elevated temperature that most other coliforms do not. Standard Methods 9222 D counts them by membrane filtration as CFU per 100 mL, and Standard Methods 9221 B, C and E1 give a most probable number by multiple-tube fermentation.

Plan collection and receipt together

Define the organism or indicator, test portion and enumeration or presence/absence question. Use the assigned bottle and collection instructions and coordinate delivery around the method requirements.

Include the complete project requirement

Provide the matrix, target analytes, required method, fraction, units and reporting limits. Include the permit or monitoring instructions where results will support a regulatory submission. Naming this method does not establish blanket compliance eligibility.

Resolve collection before sampling

Use the bottle, preservation and delivery instructions assigned to the agreed scope. Identify each location and collection time, and document any deviation. The final analytical plan must fit the submitted material and its intended use.

Related testing & guidance

Technical & service references

Vanguard service details

Source review: November 8, 2026. The agreed method and project scope govern the testing.

Start with a defined scope

Build a project scope

Tell us the sample, the question and the decision your results need to support.

Build a testing request