Testing by Vanguard Laboratory · Olympia, Washington360-967-7010

General chemistry

SM 5540 D: Surfactants (MBAS and CTAS)

Scope SM 5540 D for surfactants (mbas and ctas). Define sample matrix, preparation, reporting limits and project requirements.

Surfactants (MBAS and CTAS) measurement context

Anionic surfactants, Standard Methods 5540 C: they form a blue ion pair with methylene blue that is extracted into chloroform and measured by spectrophotometry, as methylene blue active substances (MBAS). Nonionic surfactants, Standard Methods 5540 D: they are separated from the sample by gas stripping (sublation) and measured as cobalt thiocyanate active substances (CTAS). Both results count every substance that reacts, not one named surfactant. Reported as mg/L.

Define where the property is measured

Specify field or laboratory measurement, units and the sample state needed for the project. A delivered sample may not preserve the source-time condition for a changing property.

Include the complete project requirement

Provide the matrix, target analytes, required method, fraction, units and reporting limits. Include the permit or monitoring instructions where results will support a regulatory submission. Naming this method does not establish blanket compliance eligibility.

Resolve collection before sampling

Use the bottle, preservation and delivery instructions assigned to the agreed scope. Identify each location and collection time, and document any deviation. The final analytical plan must fit the submitted material and its intended use.

Related testing & guidance

Technical & service references

Vanguard service details

Source review: November 8, 2026. The agreed method and project scope govern the testing.

Start with a defined scope

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Tell us the sample, the question and the decision your results need to support.

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