Biochemical oxygen demand (BOD) measurement context
Standard Methods 5210 B. The sample, or a dilution of it, is incubated in the dark at 20 °C for 5 days, and the drop in dissolved oxygen is the biochemical oxygen demand: the oxygen used by microorganisms breaking down the organic matter present. Carbonaceous BOD (cBOD) is the same test with a nitrification inhibitor added, so oxygen used to oxidize ammonia is not counted. Reported as mg/L of oxygen.
Connect the parameter to the sample plan
Specify grab or composite collection where applicable, the reporting unit and any field or preparation requirements. Keep nitrogen, phosphorus, solids and demand measurements on their correct reporting basis.
Include the complete project requirement
Provide the matrix, target analytes, required method, fraction, units and reporting limits. Include the permit or monitoring instructions where results will support a regulatory submission. Naming this method does not establish blanket compliance eligibility.
Resolve collection before sampling
Use the bottle, preservation and delivery instructions assigned to the agreed scope. Identify each location and collection time, and document any deviation. The final analytical plan must fit the submitted material and its intended use.
Related testing & guidance
Technical & service references
Vanguard service detailsSource review: November 8, 2026. The agreed method and project scope govern the testing.